SCN lines

Dr. Terry Niblack

Department of Crop Sciences

University of Illinois

E. mail: tniblack@uiuc.edu

Technical Report.

All inbreds (Table 1) are maintained year‑round on the appropriate host species/cultivar according to standard procedures (Niblack et al., 1993, J. Nematology Suppl. 25:880‑886) including the following: sterilized field sand medium in 1‑liter containers suspended in a 27 C water bath; fertilization with Peter's soluble 20‑20‑20; and transfer to a new host every 30‑40 days. HG Type and race testing are conducted according to established protocols once per year (Table 2), with additional characterization on an as‑needed basis. Inbreds must be requested at least 40 days before delivery to allow for each cycle of increase (30‑35 days) plus processing time. Total lead time depends on the number of nematodes requested. Inbreds for transfer are produced as described above for maintenance, except for the following: containers are 100‑ml polyvinyl chloride tubes; the infestation rate is 1,000 eggs/tube unless otherwise requested (normal yields between 100 and 400 cysts/tube); and cysts are placed in sterilized field sand and shipped via UPS.

Table 1. Isolates of Heterodera glycines available for cooperator use (2003).

		Date of	No.
Inbred	Description	origin	lines

LY1	Synthetic isolate, selected for reproduction on Hartwig by Lawrence Young	1998	1
LY2	Field isolate from Tennessee mass selected on Hartwig by Lawrence Young	1999	1
PA2	Prakash Arelli “race 2” maintained on Essex	*	1
PA3	Prakash Arelli “race 3” maintained on Essex	*	1
PA5	Prakash Arelli “race 5” maintained on Essex	*	1
OP20	Dong et al., 1997. Genetic analysis of parasitism in the soybean cyst nematode Heterodera glycines. Genetics 146:1311-1318.	*	1
OP25	Dong et al., 1997. Genetics 146:1311-1318.	*	1
OP50	Dong et al., 1997. Genetics 146:1311-1318.	*	1
TN1	gene pool isolate originally prepared by V. Dropkin	1980	1
TN2	inbred by mass selection on Tiny Tim tomato	1991	1
TN3	TN2 inbred by mass selection on Roma tomato	1991	1
TN4	TN1 inbred by mass selection on M15 lima bean	1993	1
TN5	Selected by single-cyst descent on Pickett	1988	3
TN6	Selected by single-cyst descent on Peking	1988	3
TN7	Selected by single-cyst descent on PI 88788	1988	3
TN8	Selected by single-cyst descent on PI 90763	1988	3
TN9	TN2 selected by single-cyst descent on Tiny Tim tomato	1994	11
TN10	TN3 selected by single-cyst descent on Roma tomato	1994	11
TN11	TN1 selected by single-cyst descent on Essex soybean	1994	10
TN12	TN1 inbred by mass selection on Pickett	1988	1
TN13	TN1 inbred by mass selection on Peking	1988	1
TN14	TN1 inbred by mass selection on PI 88788	1988	1
TN15	TN1 inbred by mass selection on PI 90763	1988	1
TN16	TN1 inbred by single-cyst descent on PI 209332	1996	10
TN17	TN1 selected for “fast” hatching on Essex	1998	1
TN18	TN1 selected for “slow” hatching on Essex	1998	1
TN19	LY1 selected by single-cyst descent on Hartwig	1999	10
TN20	LY1 selected by singe-cyst descent on PI 437654	1999	10
TN21	Field isolate from Missouri mass selected on Hartwig	2000	1
TN22	TN2 mass selected on PI88788	2000	1
VL1	designated Hg1 by Virgil Leudders 'high on 87008, low on 209332'	1985	1

*no date available

Table 2. Phenotypic characterization of Heterodera glycines inbred lines available for cooperator use (2002).

Isolate

No. Females on Lee 74

Female Index (FI)

HG Type

Race

PI548402

PI88788

PI90763

PI437654

PI209332

PI89772

PI548316

Pickett

LY1	140	103	99	84	80	88	82	85	1-7	138	4
LY2	126	42	29	43	12	38	49	49	1-7	103	4
OP20	418	68	25	43	0	19	67	41	1.2.3.5.6.7	76	4
OP25	203	13	8	0	0	13	0	2	1.5	45	9
OP50	280	75	30	62	0	28	87	47	1.2.3.5.6.7	78	4
PA2	251	30	14	1	0	20	3	22	1.2.5.7	116	2
PA3	200	1	3	0	0	3	0	7	0	2	3
PA5	252	25	22	1	0	19	7	44	1.2.5.7	64	2
TN1	177	11	11	1	0	16	3	27	1.2.5.7	17	2
TN2	100	32	4	10	0	5	21	2	1.3.6	54	14
TN3	198	60	14	31	0	25	46	25	1.2.3.5.6.7	112	4
TN4	100	17	9	0	0	7	2	23	1.7	32	9
TN5	139	58	17	20	0	31	19	48	1.2.3.5.6.7	96	4
TN6	154	82	14	24	0	20	42	38	1.2.3.5.6.7	113	4
TN7	117	58	75	3	0	83	5	98	1.2.5.7	120	2
TN8	179	72	8	35	0	3	53	40	1.3.6.7	97	14
TN9	144	49	0	3	0	1	10	3	1.6	55	9
TN10	132	9	6	2	0	9	7	1	0	32	6
TN11	198	8	6	3	0	4	8	0	0	19	6
TN12	112	82	17	31	0	8	58	27	1.2.3.6.7	93	4
TN13	391	75	4	41	0	5	55	31	1.3.6.7	88	14
TN14	177	23	93	0	0	63	0	59	1.2.5.7	66	2
TN15	366	95	14	74	0	8	75	57	1.2.3.6.7	118	4
TN16	283	27	89	0	0	76	1	94	1.2.5.7	69	2
TN17	127	3	6	1	0	9	2	17	7	9	3
TN18	436	4	7	1	0	11	1	26	5.7	8	3
TN19	146	84	86	71	59	75	62	65	1-7	104	4
TN20	164	60	71	51	73	115	79	132	1-7	102	4
TN21	444	46	17	30	4	22	15	44	1.2.3.5.6.7	64	4
VL1	298	9	96	0	0	74	0	86	2.5.7	25	5